Automation of plant tissue culture process
1991
Miwa, Y.
The present authors have developed a position detector and a growth state discriminator for a seedling during the previously conducted fundamental research on an automatizing plant tissue culture process. Furthermore, we have developed a micro robot for transplanting young seedling into culture medium. In this study, we will discuss a fully automated lily's bulb tissue culturing system that was developed as a trial of automation of biotechnology performed in a flower production. This system was built with integrating subsystems which are developed to perform automatically in each process of supplying a bulb, cutting its root, separating the bulbscales, transplanting bulbscales one by one, recognizing the shape of each bulbscale, and planting the bulbscale into culture medium. Individual subsystem was designed to cope with irregularity in size and shape of a bulb or bulbscale. At present, neither a virus contamination nor a detrimental effect to a genetic trait by the mechanical stressing due to the system was recognized in a trial test. It is also found that a completion of the present system was within one minute. It is, therefore, thought that the system can be used in a practical stage. Meanwhile, the culture robot of a miniature capsule enclosed structure for the home and/or personal purposes was made for a trial performance, extending our techniques towards a developing the aforementioned system. Furthermore, a protoplast positioning system using a dielectrophoresis effect in medium chamber having electrodes will be described. This system was designed to be applicable to a cell fusion and gene injection.
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