A semi-automated Raman micro-spectroscopy method for morphological and chemical characterizations of microplastic litter
2016
L., Frère | I., Paul-Pont | J., Moreau | P., Soudant | C., Lambert | A., Huvet | E., Rinnert
Every step of microplastic analysis (collection, extraction and characterization) is time-consuming, representing an obstacle to the implementation of large scale monitoring. This study proposes a semi-automated Raman micro-spectroscopy method coupled to static image analysis that allows the screening of a large quantity of microplastic in a time-effective way with minimal machine operator intervention. The method was validated using 103 particles collected at the sea surface spiked with 7 standard plastics: morphological and chemical characterization of particles was performed in <3h. The method was then applied to a larger environmental sample (n=962 particles). The identification rate was 75% and significantly decreased as a function of particle size. Microplastics represented 71% of the identified particles and significant size differences were observed: polystyrene was mainly found in the 2–5mm range (59%), polyethylene in the 1–2mm range (40%) and polypropylene in the 0.335–1mm range (42%).
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