Microchip-Based Nano Chromatography and Nano Capillary Electrophoresis in Genomics and Proteomics
2009
Ali, Imran | Aboul-Enein, Hassan Y | Gupta, Vinod K
The growing interest in proteomics and genomics needs analytical techniques capable of detecting proteins and nucleic acids at the ng L⁻¹ levels due to the small amounts of various proteins and nucleic acids in living cells. During the last decade remarkable development has occurred in the evolution of chromatographic and capillary electrophotetic methods on microchips. These methods are called nano liquid chromatography (NLC) and nano capillary electrophoresis (NCE). The present review article describes the application of NLC and NCE to proteomics and genomics research The analyses of proteins and nucleic acids at low levels is described together with the optimization and future trends of nano methods in genomics and proteomics.
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