Enrichment and separation of chlorogenic acid from the extract of Eupatorium adenophorum Spreng by macroporous resin
2016
Liu, Boyan | Dong, Beitao | Yuan, Xiaofan | Kuang, Qirong | Zhao, Qingsheng | Yang, Mei | Liu, Jie | Zhao, Bing
A simple and efficient chromatographic method for separation of chlorogenic acid from Eupatorium adenophorum Spreng extract was developed. The adsorption properties of nine macroporous resins were evaluated. NKA-II resin showed much better adsorption/desorption properties. The adsorption of chlorogenic acid on NKA-II resin at 25°C was well fitted to Langmuir isotherm model and pseudo-second-order kinetic model. The dynamic adsorption and desorption experiments were carried out on columns packed with NKA-II resin to optimize the separation process. The content of chlorogenic acid in the product increased to 22.17%, with a recovery yield of 82.41%.
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