Description of Massilia rubra sp. nov., Massilia aquatica sp. nov., Massilia mucilaginosa sp. nov., Massilia frigida sp. nov., and one Massilia genomospecies isolated from Antarctic streams, lakes and regoliths
2020
Holochová, Pavla | Mašlaňová, Ivana | Sedláček, Ivo | Švec, Pavel | Králová, Stanislava | Kovařovic, Vojtěch | Busse, Hans-Jürgen | Staňková, Eva | Barták, Miloš | Pantůček, Roman
Bacteria of the genus Massilia often colonize extreme ecosystems, however, a detailed study of the massilias from the Antarctic environment has not yet been performed. Here, sixty-four Gram-stain-negative, aerobic, motile rods isolated from different environmental samples on James Ross Island (Antarctica) were subjected to a polyphasic taxonomic study. The psychrophilic isolates exhibited slowly growing, moderately slimy colonies revealing bold pink-red pigmentation on R2A agar. The set of strains exhibited the highest 16S rRNA gene sequence similarities (99.5–99.9%) to Massilia violaceinigra B2ᵀ and Massilia atriviolacea SODᵀ and formed several phylogenetic groups based on the analysis of gyrB and lepA genes. Phenotypic characteristics allowed four of them to be distinguished from each other and from their closest relatives. Compared to the nearest phylogenetic neighbours the set of six genome-sequenced representatives exhibited considerable phylogenetic distance at the whole-genome level. Bioinformatic analysis of the genomic sequences revealed a high number of putative genes involved in oxidative stress response, heavy-metal resistance, bacteriocin production, the presence of putative genes involved in nitrogen metabolism and auxin biosynthesis. The identification of putative genes encoding aromatic dioxygenases suggests the biotechnology potential of the strains. Based on these results four novel species and one genomospecies of the genus Massilia are described and named Massilia rubra sp. nov. (P3094ᵀ=CCM 8692ᵀ=LMG 31213ᵀ), Massilia aquatica sp. nov. (P3165ᵀ=CCM 8693ᵀ=LMG 31211ᵀ), Massilia mucilaginosa sp. nov. (P5902ᵀ=CCM 8733ᵀ=LMG 31210ᵀ), and Massilia frigida sp. nov. (P5534ᵀ=CCM 8695ᵀ=LMG 31212ᵀ).
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