Effectiveness of talc as adsorbent for purification and immobilization of plant lipoxygenases
1994
Battu, S. | Rabinovitch-Chable, H. | Beneytout, J.L.
The present study describes the immobilization of lipoxygenase from Solanum tuberosum tuber and Lupinus albus, Cicer arietinum, and Pisum sativum seeds on a magnesium silicate based support, talc. In all cases, an increase in specific activity of many hundreds of units per milligram was obtained and the use of free or immobilized enzymes was quite similar. The adsorption of plant lipoxygenases on talc was a direct, rapid, and inexpensive method to purify and stabilize these enzymes.
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