Production, characteristics and applications of phytase from a rhizosphere isolated Enterobacter sp. ACSS
2016
Chanderman, Ashira | Puri, Adarsh Kumar | Permaul, Kugen | Singh, Suren
Optimization of process parameters for phytase production by Enterobacter sp. ACSS led to a 4.6-fold improvement in submerged fermentation, which was enhanced further in fed-batch fermentation. The purified 62 kDa monomeric phytase was optimally active at pH 2.5 and 60 °C and retained activity over a wide range of temperature (40–80 °C) and pH (2.0–6.0) with a half-life of 11.3 min at 80 °C. The kinetic parameters K ₘ, V ₘₐₓ, K cₐₜ, and K cₐₜ/K ₘ of the pure phytase were 0.21 mM, 131.58 nmol mg⁻¹ s⁻¹, 1.64 × 10³ s⁻¹, and 7.81 × 10⁶ M⁻¹ s⁻¹, respectively. The enzyme was fairly stable in the presence of pepsin under physiological conditions. It was stimulated by Ca⁺², Mg⁺² and Mn⁺², but inhibited by Zn⁺², Cu⁺², Fe⁺², Pb⁺², Ba⁺² and surfactants. The enzyme can be applied in dephytinizing animal feeds, and the baking industry.
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