Induction of cytochrome P450 4A1 in mouse liver by the herbicide synergist tridiphane
1992
Levi, P.E. | Rose, R.L. | Adams, N.H. | Hodgson, E.
The herbicide synergist tridiphane [2-(3,5-dichlorophenyl)-2-(2,2,2,-trichloroethyl) oxirane] was examined for its ability to induce cytochrome P450 in vivo. Male C57BL/6N mice were given tridiphane, 250 mg/kg ip, for 3 days. Liver weight and P450 content were increased after tridiphane treatment, and SDS-PAGE showed an increase in microsomal proteins in the 50-60 kDa range. No significant increases in enzymatic activities were observed with the substrates benzphetamine, p-nitroanisole, benzo[a]pyrene, or ethoxyresorufin. There was, however, a 10-fold elevation in microsomal hydroxylation of lauric acid, an activity specifically associated with induction of P450 4A1. Western blot analysis using an antibody specific for P450 4A1 showed a dramatic increase in P450 4A1.
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