Research on the regulatory mechanisms of self-incompatibility plasticity in Nicotiana alata
2020
Liao, Jugou | Wei, Xuemei | Chen, Zhiyun | Qin, Xiaojun | Pan, Zihui | Niu, Yongzhi | Suo, Wenlong | Zheng, Yunye | Ma, Wenguang | Chen, Suiyun
Nicotiana alata is a model species used to study gametophytic self-incompatibility (SI), controlled by the S-locus (encoding S-RNase and SLF proteins expressed in pistil and pollen, respectively) as well as by modifier genes (such as 120 kDa, HT-b and Trxh). A previous study revealed that SI in N. alata accession S₂S₂ was plastic, and that SI was leaky with senescence of the pistil and plant. Here, we further explored its regulatory mechanisms. Reverse transcription quantitative PCR (RT-qPCR) and western blot results showed that during the senescence of pistil in S₂S₂, 120 kDa and HT-b were transcribed and translated at lower levels, while marked residual S-RNase activity was detected. The transcripts of Trxh varied irregularly. Only the transcription and translation of 120 kDa declined with the plant senescence. According to the results, PSF in S₂S₂ may be associated with down-regulated expression of 120 kDa and HT-b, while S-RNase and Trxh seem to be unrelated to this phenotype. These findings deepen our understanding of the regulatory mechanisms of SI plasticity in N. alata.
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