Predictability of combined effects of eight chloroacetanilide herbicides on algal reproduction

Chloroacetanilides are pre-emergence herbicides for the control of annual grasses and broad-leaf weeds. As a result of their extensive use, residues are often found in surface waters. Observed simultaneous occurrence of different chloroacetanilide herbicides gives reason for concern about potential combination effects on aquatic non-target organisms. This study aimed to clarify whether joint effects of various chloroacetanilide herbicides may be predictable from knowledge of concentration–response relationships of single substances. Whether the chloroacetanilides all share the same mode of action is unclear. Therefore we investigated the predictive value of two alternative concepts for the prediction of combined effects: concentration addition, which assumes a similar mode of action, and independent action, which is based on the idea of a dissimilar mode of action of the mixture components. Eight chloroacetanilides (acetochlor, alachlor, butachlor, dimethachlor, metazachlor, metolachlor, pretilachlor and propachlor) were experimentally tested for their individual as well as for their combined effects in mixtures on the reproduction of the green alga Scenedesmus vacuolatus. Individual chloroacetanilides impaired algal reproduction, with EC50 values ranging from 3 to 232 µg litre−1. The differences in EC50 values were strongly correlated with the lipophilicities of the compounds. Effects of chloroacetanilide mixtures were considerably higher than those of the individual components: a complete inhibition of algal reproduction was observed when every mixture component was present in a concentration that would cause only 5% effect if applied singly. However, the combined effects proved to be predictable by using the concept of concentration addition. The alternative concept, independent action, distinctly underestimated the mixture toxicity. These findings (1) indicate a similar mechanism of action of chloroacetanilides in algae and (2) reinforce the view that concentration addition is a reasonable assumption for the predictive hazard assessment of groups of similarly acting herbicides.