In vitro maturation and fertilization of swamp buffalo oocytes and their subsequent development
1992
Pavasuthipaisit, K. | Kitiyanant, Y. | Thonabulsombat, C. | Tocharus, C. | Sriurairatna, S. | White, K.L.
The present experiment was carried out to evaluate the maturation, fertilization and subsequent embryo culture of swamp buffalo oocytes in vitro. The oocytes (n = 273) were collected and morphologically graded based on the structure of cumulus-oocyte complexes as Grade 1 (compact, n = 81), Grade 2 (expanded, n = 70), Grade 3 (partially denuded, n = 65) or Grade 4 (completely denuded, n = 57). More than 60% of the in vitro matured oocytes co-cultured with capacitated spermatozoa demonstrated evidence of fertilization or cleavage to the 2-cell stage when either Grade 1 or 2 oocytes were used. The percentage of fertilized oocytes undergoing 2-cell stage cleavages from Grade 3 (53%) and Grade 4 (46%) groups was significantly lower (P < 0.01) than that observed in the Grade 1 (64%) and Grade 2 (68%) groups. Development to the 6 to 8 cell stage substantiated fertilization of Grade 1 and 2 oocytes. These results demonstrated that swamp buffalo oocytes are capable of maturing in vitro, forming embryos, and developing at least to the 8-cell stage in culture medium alone.
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