Larvicidal activity of lectins on Lucilia cuprina: mechanism of action
1994
Eisemann, C.H. | Donaldson, R.A. | Pearson, R.D. | Cadogan, L.C. | Vuocolo, T. | Tellam, R.L.
Larvae of the blowfly Lucilia cuprina (Wied.) (Diptera: Calliphoridae) were grown in vitro on a serum-free medium in the presence of a number of lectins. Lectins with specificities for β-(1,4)-N-acetylglucosamine (wheat germ lectin) and α-D-mannopyranosyl and (α-D-glucopyranosyl residues (lentil lectin and Con A) caused strong concentration-dependent inhibition of the growth of the larvae and substantial mortality. Wheat germ lectin had the strongest effects, showing 50% inhibition of larval growth at a concentration of 2 μM and 100% mortality at 25 μM. Other lectins with different sugar specificities had much less effect. The mechanism of the larvicidal action(s) of wheat germ lectin, lentil lectin and Con A was investigated. There were at least three effects of these lectins on L. cuprina larvae. First, these lectins bound to and reduced the permeability of the peritrophic membrane of the larvae. Second, they reduced ingestion of diet medium by larvae. Third, the lectins also bound to the apical membranes of larval gut epithelial cells although there were no obvious signs of damage to these cells. It is concluded that the combination of these effects probably results in the starvation of the larvae. The implications of these results in terms of possible control strategies for L. cuprina are discussed.
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