Isolation and Expression Profiling of Insecticidal Lectins from Wild Alliums Against Onion Thrips (Thrips tabaci Lindeman)
2022
Khandagale, Kiran | Roylawar, Praveen | Randive, Pragati | Karuppaiah, Vadivelu | Soumia, Parakkattu Sulochanan | Shirsat, Dhananjay | Gedam, Pranjali | Ade, Avinash | Gawande, Suresh | Mejara Siṅgha,
Lectins are important for plant defence against various insect and viral invasions. Allium species are rich source of lectins, but till date no lectin is reported to have insecticidal activity against the onion thrips. In the present study, wild Allium species were mined for potential lectins against onion thrips. The full-length lectin genes were amplified and cloned from four wild Allium species (A. hookeri, A. altaicum, A. fistulosum and A. angulosum) which is 534 bp in length. The ‘QXDXNXVXY’ motif responsible for recognition of alpha-D-mannose is present in all the sequences of lectin. The qPCR expression analysis of lectin genes showed that A. hookeri has fourfold more expression as compared to the onion (Allium cepa). The whole plant damage assay and detached leaf damage assay revealed that A. hookeri was resistant to Thrips tabaci. The per cent leaf area damage was lowest in A. hookeri, and further larval survival rate of T. tabaci was also least (61.46 ± 1.47%) when fed on A. hookeri. The overall development of T. tabaci was also found to be delayed on A. hookeri compared to other species. The present investigation will be helpful in developing interspecific crosses and transgenics for thrips control in onion.
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