An affinity capture involved enzymatic assay for thrombin by using peptide aptamers as affinity ligands on magnetic beads
2013
Zhao, Qiang | Gao, Jie
Here we present a sensitive and specific assay for thrombin through the affinity capture of thrombin with mRNA display generated peptide aptamers on magnetic beads and the subsequent thrombin-involved enzymatic reaction. Thrombin at 50 fM can be detected.
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Bibliographic information
Chemical communications
                                                                
                                                                
                                                                    Volume
                                                                    49
                                                                
                                                                
                                                                
                                                                    Issue
                                                                    70
                                                                
                                                                
                                                                
                                                                    
                                                                    Pagination
                                                                    7720
                                                                    
                                                                    
                                                                        - 7722
                                                                    
                                                                
                                                                
                                                                
                                                                
                                                                
                                                                    ISSN
                                                                    1364-548X
                                                                
                                                            Publisher
                                            The Royal Society of Chemistry
                                        Other Subjects
                                            Enzymatic reactions; Oligonucleotides; Thrombin
                                        Language
                                            English
                                        Type
                                            Journal Article; Text
                                        
                                            
                                            2024-02-28
                                        
                                        
                                        
                                        
                                            
                                            MODS
                                        
                                    
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