Increasing erucic acid content in Ethiopian mustard through mutation breeding
1998
VELASCO, L. | FERNÁNDEZ‐MARTINEZ, J. M. | HARO, A de
Ethylmethane sulphonate (EMS) was applied to seeds of the Ethiopian mustard (Brassica carinata A. Braun) line C‐101. Bulk samples of M₃ seeds from 8331 M₂ plants were evaluated for the fatty acid composition of their oil by near‐infrared reflectance spectroscopy (NIRS) and by further gas chromatography on selected samples. A putative mutant, N2‐6230, showing very low oleic acid content (4.7% vs. average of 8.6% in C‐101) and erucic acid content within the range of variation of the line C‐101 (40‐49.3%) was identified. The M₃ progeny of this mutant showed a wide segregation for erucic acid content (39.1‐57.9% vs. 41.8‐50.3% in C‐101), and maintained levels of oleic acid lower than in line C‐101. Selection for high erucic acid content in the M₃ and M₄ generations led to the fixation of this mutation in the M₅ generation (52.2‐59.3% vs. 39.0‐47.6% in C‐101). This is the first high erucic acid line obtained in Brassica species through mutation breeding. Its utility in future programmes to develop very high erucic acid lines is discussed.
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