A proton nuclear magnetic resonance method for the quantitative analysis on a dry weight basis of (1 leads to 3)-beta-D-glucans in a complex, solvent-wet matrix
2001
Lowman, D.W. | Williams, D.L.
Health benefits of the polysaccharide (1 leads to 3)-beta-D-glucan, reported to induce immunobiological, hypocholesterolemic, and hypoglycemic effects in humans and animals, have made the isolation, characterization, and assay of a viable glucan product critical. A new analytical method, based on internal standard proton NMR analysis, for the assay of solvent-wet samples containing (1 leads to 3)-beta-D-glucan is presented. The method enables glucan identification, provides a solvent-free assay, and improves upon the previous multistep extraction and lyophilization procedure by reducing the 1-2 day analysis time to 1-2 h. NMR offers a rapid method for quantifying the glucan in commercial samples, such as nutraceuticals, as well as industrial samples enabling better evaluation of the efficacy of these carbohydrates in health-related applications.
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