Identification of Components of Traditional Chinese Medicine Preparation “Jing-Zhi-Guan-Xin” Troche by Medium Pressure LC and HPLC-DAD-MS
2007
Li, Yunfei | Liu, Li | Cheng, Yiyu
Comprehensive identification of the phytochemical components is one of the key points in the study on traditional Chinese medicine (TCM). In the present study, an approach combining separation and identification of the complex chemical composition in a TCM preparation named “Jing-Zhi-Guan-Xin” (JZGX) troche was developed. Medium pressure liquid chromatography (MPLC) was used to separate JZGX troche into several fractions according to their different polarity. Then, HPLC-DAD-MS was performed to acquire MS and UV spectra of the components within each of the separated fractions. Finally, 64 components were detected, among which, 22 components were identified by comparing their obtained retention times, molecular weights and UV spectra with the available standards and reference data. The results indicate that this approach is beneficial to explore chemical composition of traditional Chinese medicine preparation.
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