A universal method for automated gene mapping
2005
Zipperlen, Peder | Nairz, Knud | Rimann, Ivo | Basler, Konrad | Hafen, Ernst | Hengartner, Michael | Hajnal, Alex
Small insertions or deletions (InDels) constitute a ubiquituous class of sequence polymorphisms found in eukaryotic genomes. Here, we present an automated high-throughput genotyping method that relies on the detection of fragment-length polymorphisms (FLPs) caused by InDels. The protocol utilizes standard sequencers and genotyping software. We have established genome-wide FLP maps for both Caenorhabditis elegans and Drosophila melanogaster that facilitate genetic mapping with a minimum of manual input and at comparatively low cost.
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