Covalent immobilization of ascorbate oxidase onto polycarbonate strip for l-ascorbic acid detection
2012
Kannoujia, Dileep Kumar | Kumar, Saroj | Nahar, Pradip
Herein, a simple and rapid method is described for detection of l-ascorbic acid by ascorbate oxidase immobilized onto polycarbonate strip pre-activated by 1-fluoro-2-nitro-4-azidobenzene in photochemical reaction. Covalent attachment of ascorbate oxidase was confirmed by XPS studies. The immobilized-ascorbate oxidase shows higher pH, thermal and storage stability in comparison to free enzyme.
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