Central and peripheral concentrations of tumor necrosis factor-α in Chinese Meishan pigs stimulated with lipopolysaccharide
2003
Sakumoto, R. | Kasuya, E. | Komatsu, T. | Akita, T.
The objective of this study was to investigate the presence of tumor necrosis factor-α (TNF-α) in the central nervous system and the effects of lipopolysaccharide on central and peripheral concentrations of TNF-α, behavioral conditions (standing or lying), elimination scores (defecation or urination), rectal temperature, and food intake (as-fed basis) in Chinese Meishan pigs. Intravenous injections of lipopolysaccharide resulted in increased (P < 0.05) plasma concentrations of TNF-α and cortisol. Although urination was not affected by the administration of lipopolysaccharide, defecation was stimulated (P < 0.05). Lipopolysaccharide increased (P < 0.05) rectal temperature and standing rate, and inhibited (P < 0.05) food intake in pigs. To determine whether TNF-α is present in the porcine central nervous system as well as in peripheral blood, TNF-α and its specific transcripts in brain tissues (hypothalamus, amygdala, or hippocampus) and the pituitary were determined. The abundance of TNF-α messenger RNA and immunoreactive TNF-α were observed in all tissues, and the concentrations of TNF-α were increased (P < 0.05) by the intramuscular injection of lipopolysaccharide. These results suggest that TNF-α is present in the central nervous system, and plays some roles in its biological regulation in Chinese Meishan pigs.
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