Isolation and Characterization of Phosphate Solubilizing Bacteria from Rhizosphere of Dioscorea alata Stimulating Growth and Diosgenin Production
2017
Pandey, Devendra Kumar | Nazir, Adil | Dey, Abhijit
The effect of phosphate-solubilizing bacteria (PSB), Bacillus subtilis KF279045, identified on the basis of 16S rRNA gene sequencing was investigated on plant growth and diosgenin content in Dioscorea alata in pots containing tricalcium phosphate (TCP) supplemented soil. The PSB were isolated from the rhizosphere of D. alata plants and tested for P-solubilization ability and indole acetic acid production. High performance thin layer chromatography method was used for quantitative determination of diosgenin in different samples of D. alata. In glasshouse study, treatment of PSB resulted in increased plant growth and diosgenin content. The stimulatory effect was observed with PSB + TCP treatment in plant growth parameters and diosgenin content was compared to the uninoculated plants. This is the first study documenting the stimulatory effect of PSB on diosgenin content and it suggests the establishment of synergistic interactions between B. subtilis and D. alata.
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