Bioprospecting thelat gene in soil samples
2003
Dharwadkar, Aarohi | Gupta, Vidya | Pant, Aditi
Twenty soil communities from the northeastern forests (Assam) and the Western Ghats (Maharashtra) were screened for the presence of the lysine aminotransferase (lat) gene fromNocardia. Hybridization probes and primers were synthesized in accordance with the reported sequence of theNocardia lat gene from GenBank (number: G1 49355). Seven positives were obtained from the 20 soils. Six of the seven positive were from the Western Ghats and one from the northeast Assam forests. Eighteen actinomycete isolates from the 7 positive soils showed the presence of thelat gene. Only 9 isolates actually produced an antibiotic. These results are discussed.
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