Effect of Temperature and Light Exposure on the Detection of Total Intact Glucosinolate Content by LC-ESI-MS in Broccoli Leaves
2014
Ares, Ana M. | Nozal, María J. | Bernal, José L. | Bernal, José
Total intact glucosinolate content in broccoli leaf extracts (Ramoso calabrese cultivar) has been determined by liquid chromatography coupled to tandem mass spectrometry with the aim of detecting potential differences in this value due to the effects of the drying temperature or the working and storage conditions (temperature and light exposure). Those broccoli leaf extracts were obtained with two different sample treatments based on heating the sample (microwave or oven), and using boiling water as extraction solvent. Significant differences were observed in the total intact glucosinolate content depending on the drying temperature and light exposure under typical working conditions. Meanwhile, those differences were less remarkable when the extracts were stored at low temperature and protected from light exposure.
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