Assessment of VITEK® 2, MALDI-TOF MS and full gene 16S rRNA sequencing for aerobic endospore-forming bacteria isolated from a pharmaceutical facility
2022
Costa, Luciana Veloso da | Miranda, Rebeca Vitoria da Silva Lage de | Fonseca, Erica Louro da | Gonçalves, Natalia Pedra | Reis, Cristhiane Moura Falavina dos | Frazão, Adriana Marques | Cruz, Fernanda Ventura | Brandão, Marcelo Luiz Lima | Ramos, Juliana Nunes | Vieira, Verônica Viana
VITEK®2, MALDI-TOF MS and 16S rRNA sequencing were evaluated for the identification of aerobic endospore-forming bacteria (AEB) from a pharmaceutical facility. MALDI-TOF MS demonstrated higher accuracy compared to VITEK®2, although both databases were insufficient to identify AEB species. Sequencing was the best methodology, but unable to identify closely related species.
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