Inhibitory effect of chlorogenic acid on polyphenol oxidase and browning of fresh-cut potatoes
2020
Zheng, Dai | Wang, Guangliang | Tang, Jinlei | Yao, Congying | Li, Pengfei | Song, Qi | Wang, Chunling
In our study, the inhibition mechanism of chlorogenic acid (CGA) on PPO was investigated by enzyme kinetic analysis, fluorescence quenching, thermodynamic parameters analysis, circular dichroism and molecular docking. Our results showed that CGA inhibited PPO activity in a reversible mixed-type manner and Kᵢ was estimated to be 3.577 × 10⁻⁴ mol L⁻¹. PPO activity was significantly inactivated by CGA in a dose-dependent manner and IC₅₀ was calculated as 1.55 × 10⁻⁴ mol L⁻¹. CGA interacts with PPO and quench its intrinsic fluorescence. Furthermore, results indicated that CGA bound with PPO by hydrophobic interaction. In addition, CGA changed the hydrogen bonding network of PPO and resulted in rearragement of secondary structure. The molecular docking results suggested that CGA bound to active site of PPO. Importantly, the efficacy of CGA inhibiting the enzymatic browning of fresh-cut potato slices was confirmed by the inhibition of PPO. Therefore, based on the inhibition of PPO, CGA might represent a new type of inhibitor for fresh-cut potatoes.
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