A glycoprotein highly toxic for Galleria mellonella larvae secreted by the entomopathogenic fungus Beauveria sulfurescens
1994
Mollier, P. | Lagnel, J. | Fournet, B. | Aioun, A. | Riba, G.
The culture filtrate of the hyphomycete fungus Beauueria sulfurescens contains several types of toxin. They cause different symptoms when injected into the hemocoel of Galleria mellonella larvae and display different chromatographic properties. One of these toxins was partially purified by a three-step fractionation procedure. The toxic fraction contained only 0.075% of the protein of the crude extract, indicating that the toxic component was present at very low concentration in the culture filtrate of B. sulfurescens. The apparent molecular weight of the toxin was between 10 and 29 X 10(4). The toxin is a N-linked glycoprotein with oligomannosidic-type sugar chains. It displayed no proteolytic activity and appeared to be extremely potent on G. mellonella larvae: its LD50 was less than 10 microgram/kg of larvae. The injection of the toxin into larvae was accompanied by variable insect cuticle melanization.
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