Comparison of protein profiles during cotton (Gossypium hirsutum L.) fiber cell development with partial sequences of two proteins
1996
Ferguson, D.L. | Turley, R.B. | Triplett, B.A. | Meredith, W.R. Jr
Two-dimensional polyacrylamide gel electrophoresis was used to compare the protein profiles of cotton (Gossypium hirsutum L.) fiber cells at two developmental stages. In the first stage, the cells are elongating and synthesizing primary cell walls [14 days postanthesis (DPA)]; in the second stage, the cells are beginning secondary cell wall synthesis [21 DPA]. Forty-five proteins were found that differed in amount between the two stages; these proteins were characterized by their molecular weights, isoelectric points, and relative quantities at each stage. Two of the proteins that were present in higher amounts in the 21-DPA fiber were further studied by N-terminal amino acid sequencing. One of these proteins was identified as a cytoplasmic malate dehydrogenase on the basis of N-terminal sequence similarity. These results provide a foundation for further studies of the proteins that participate in cotton fiber development.
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