Rhizobium deserti sp. Nov Isolated from Biological Soil Crusts Collected at Mu Us Sandy Land, China
2020
Liu, Lei | Liang, Lixiong | Xu, Lijun | Chi, Miao | Zhang, Xiaoxia | Li, Lubin
A novel gram-negative, aerobic, non-spore-forming, rod-shaped, and non-nitrogen-fixing bacterium, named SPY-1ᵀ, was isolated from biological soil crusts collected at Mu Us Sandy Land, China. Based on 16S rRNA sequence similarity, strain SPY-1ᵀ was most closely related to Neorhizobium alkalisoli CCTCC AB 2014138ᵀ (98.7%), Neorhizobium huautlense CGMCC 1.2538ᵀ (98.6%), Neorhizobium galegae DSM 11542ᵀ (98.4%), Rhizobium wenxiniae 166ᵀ (97.9%), and Rhizobium smilacinae CCTCC AB 2013016ᵀ (97.5%). Phylogenetic analysis based on 16S rRNA sequencing and multilocus sequence analysis of partial sequences of atpD-glnII-glnA-recA-ropD-thrC housekeeping genes both indicated that strain SPY-1ᵀ was a member of the genus Rhizobium. The draft genome of strain SPY-1ᵀ was 4.75 Mb in size, and the G + C content was 60.0%. The average nucleotide identity (ANI) values to N. alkalisoli CCTCC AB 2014138ᵀ and R. smilacinae CCTCC AB 2013016ᵀ were both 84.0%. The digital DNA–DNA hybridization (dDDH) values to N. alkalisoli CCTCC AB 2014138ᵀ and R. smilacinae CCTCC AB 2013016ᵀ were 20.9% and 20.2%, respectively. The major cellular fatty acids were summed feature 8 (C₁₈:₁ω7c and/or C₁₈:₁ω6c) and C₁₆:₀. Based on the data from chemotaxonomic, phylogenetic, and phenotypic evidence, strain SPY-1ᵀ represents a novel species in the genus Rhizobium, for which the name Rhizobium deserti sp. nov. is proposed. The type strain is SPY-1ᵀ (= ACCC 61627ᵀ = JCM 33732ᵀ).
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