Alterations in Ecdysone Content during the Post-Embryonic Development of Chironomus thummi: Correlations with Chromosomal Puffing
2014
Valentin, M. | Bollenbacher, W. E. | Gilbert, L. I. | Kroeger, H.
The ecdysone titer of larvae and pupae of Chironomus thummi was determined by radioimmune assay (RIA) and revealed a concentration of about 150 ng/g fresh weigth in late third instar larvae and a peak of more than 450 ng/g fresh weight just preceding pupation. The ecdysone titer curve shows a high degree of correlation with previously observed puffing activity at the ecdysone sensitive chromosomal site IIIdl. Further, β-ecdysone is the only endogenous ecdysone detected by high pressure liquid chromatography during larval-pupal development. It was also observed that developmentally arrested Chironomus contain less ecdysone than “normal” larvae.
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