Development of a single-plate combined indirect ELISA (CI-ELISA) for the detection of antibodies against peste-des-petits-ruminants and bluetongue viruses in goats
2015
Yousuf, Raja Wasim | Sen, Arnab | Mondal, Bimalendu | Biswas, Sanchay Kumar | Chand, Karam | Rajak, Kaushal Kishore | Gowane, Gopal R. | Sudhakar, Shashi Bhusan | Pandey, A. B. (Awadh Bihari) | Ramakrishnan, Muthannan Andavar | Muthuchelvan, Dhanavelu
Peste-des-petits-ruminants and bluetongue are two of the most important viral diseases of small ruminants. In the present study, a combined indirect enzyme-linked immunosorbent assay (CI-ELISA) was developed, which can be run parallelly on the same plate for simultaneous detection of antibodies against peste-des-petits-ruminants virus and bluetongue virus. A total of 503 goat sera samples were used for developing the CI-ELISA. The relative sensitivity and specificity of CI-ELISA for detection of antibodies against PPRV were 90% and 88.8%, respectively at cut off level of 34.52% positivity. In case of BTV, 95.9% relative sensitivity and 98.6% specificity values were obtained at cut-off level of 40.42% positivity. These results indicate that the single plate CI-ELISA reported here could be used as an alternate method to existing dual plate competitive ELISAs for diagnosis/sero-surveillance of both the diseases.
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