BMP‐15 activity on in vitro development of collared peccary (Pecari tajacu Linnaeus, 1758) preantral follicles
2020
Gomes, Henrique A. N. | Campos, Lívia B. | Praxedes, Érica C. G. | Oliveira, Moacir F. | Pereira, Alexsandra F. | Silva, Alexandre R. | Saraiva, Márcia V. A.
This study investigated the effects of BMP‐15 on the in vitro development of preantral follicles of collared peccaries. Ovarian fragments were cultured for 1 or 6 days in Tissue Culture Medium 199 (TCM199⁺) supplemented with BMP‐15 at rates of 0, 1, 25 or 50 ng/ml. The fragments were analysed histologically by evaluating follicular morphology, activation and growth as well as the potential for proliferation of granulosa cells. Our results show the addition of 25 ng/ml BMP‐15 in the medium provided the greatest percentage of normal follicles (79.67% ± 0.69) when compared to other treatments (p < .05); however, this result is similar to 1 ng/ml BMP‐15 (74.00% ± 1.90, p > .05). Moreover, 25 and 50 ng/ml of BMP‐15 promoted follicular activation. BMP‐15 supplements did not affect oocyte and follicular growth. All concentrations of BMP‐15 increased the number of nucleolus organizer regions (NORs) after 1 day of culture when compared to fresh fragments or the control samples (p < .05). However, at the end of the experiment, the number of NORs in follicles cultured in all treatments was higher than that observed in the fresh control (sample taken prior to culturing) (p > .05). In summary, the addition of 25 ng/ml BMP‐15 to the culture medium of collared peccary preantral follicles maintained a high number of morphologically healthy follicles and stimulated the activation of primordial follicles after 6 days in culture.
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