Discovering peach QTLs with multiple progeny analysis
2017
Hernandez Mora, J. R. | Micheletti, D. | Bink, M. C. A. M. | Weg, W. E van de | Bassi, D. | Nazzicari, N. | Caprera, A. | Dettori, M. T. | Micali, S. | Dirlewanger, E. | Lambert, P. | Pascal, T. | Banchi, E. | Troggio, M. | Rossini, L. | Verde, I. | Quilot-Turion, B. | Laurens, F. | Arús, Pere | Aranzana, M. J.
Although many peach QTLs responsible for the variability of traits have been identified and published so far, the number of molecular markers currently used in breeding is still limited. One of the reasons is the large QTL intervals produced, in part, by the limited progeny size. Here we report a QTL mapping approach that enlarges the progeny size by analyzing jointly multiple progenies. Our analysis included 1467 individuals from eighteen Prunus progenies, 13 from intra-specific crosses and five inter-specific between peach and closely related species. The progenies were grown in five locations, with no duplication between orchards. Data from phenology, tree, flower and fruit traits, fruit quality and yield measured in different locations and years were subjected to different standardization methods and integrated in a single data file. The populations were genotyped with the 9K SNP Illumina array, which increased considerably the marker density compared to previous studies. The QTL analysis was conducted with FlexQTLTM software. Here we describe and discuss the preliminary QTLs obtained for some of the traits analyzed (maturity date, percentage of red skin color and soluble solid content). The identification of donors of favorable alleles will represent an important tool for marker-assisted breeding. This study has been conducted in the frame of the Fruit Breedomics European project.
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