Mapping local electric fields in proteins at biomimetic interfaces
2011
Schkolnik, Gal | Utesch, Tillmann | Salewski, Johannes | Tenger, Katalin | Millo, Diego | Kranich, Anja | Zebger, Ingo | Schulz, Claudia | Zimányi, László | Rákhely, Gábor | Mroginski, Maria Andrea | Hildebrandt, Peter
We present a novel approach for determining the strength of the electric field experienced by proteins immobilised on membrane models. It is based on the vibrational Stark effect of a nitrile label introduced at different positions on engineered proteins and monitored by surface enhanced infrared absorption spectroscopy.
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