Effects of the medium ammonium-nitrate ratio on competence for asparagus cell division induced by phytosulfokine-alpha
1998
Matsubayashi, Y. | Sakagami, Y.
The effects of medium ammonium-nitrate ratio on cell proliferation were investigated using a low cell-density culture of Asparagus officinalis L., which was trigered by a peptidal plant growth factor, phytosulfokine-alpha (PSK-alpha). The asparagus cells proliferated the most in a medium with an ammonium-nitrate ratio of 0:30 mM and could be maintained without significant loss of responsiveness to PSK-alpha for at least 96 h from the beginning of culture. In this medium, single cells gave rise to microcalli at initial densities as low as 3.2x10(2) cells/ml as long as PSK-alpha was present in the medium. Increasing the ammonium-nitrate ratio resulted in severe inhibition of cell proliferation at a low cell density, even if PSK-alpha was added to the medium.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by National Agricultural Library