Adhesion of K99 fimbriated Escherichia coli to pig intestinal epithelium: correlation of adhesive and non-adhesive phenotypes with the sialoglycolipid content
1991
Seignole, D. | Mouricout, M. | Duval-Iflah, Y. | Quintard, B. | Julien, R.
Evidence for the existence of two phenotypes of piglets born to experimental herds was obtained based on the susceptibility of intestinal brush borders to adhesion of K99-positive Escherichia coli. The enterocytes of the K99-receptive piglets displayed a characteristic sialoglycolipid pattern, with a higher content of the monosialoglycolipids II3 NeuGc-LacCer (GM3Gc), IV3 NeuGc-nLcOse4Cer (SPGGc) and IV3 NeuAc-nLcOse4Cer (SPG) and the oligosialogangliosides IV3 NeuAc,II3 NeuAc-GgOse4Cer (GD1a), II3 (NeuAc)2-GgOse3Cer (GD2), II3 (NeuAc)2-GgOse4Cer (GD1b) and IV3 NeuAc,II3 (NeuAc)2-GgOse4Cer (GT1b) when compared to the gangliosides of non-receptive piglets. The gangliosides from enterocytes of the non-receptive piglets were mainly the monosialogangliosides II3 NeuAc-GgOse3Cer (GM2) and II3 NeuAc-LacCer (GM3), only traces of the other sialoglycolipids being detected. Adhesion of 14C-labelled K99-positive E. coli cells to the piglet small intestinal sialoglycolipids, as tested by the thin-layer chromatogram overlay assay, revealed that the receptive enterocyte membrane was richer in glycolipids containing K99 receptor structures than the non-receptive enterocyte. Adhesion of K99-positive E. coli correlated with the degree of sialylation of the brush border glycolipids.
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