Large-scale clonal propagation of Bambusa balcooa Roxb.: An industrially important bamboo species
2020
Rajput, Bharat S. | Jani, Minal | Ramesh, Kasuladev | Manokari, M | Jogam, Phanikanth | Allini, Venkateswar Rao | Kher, Mafatlal M. | Shekhawat, Mahipal S.
Bambusa balcooa Roxb. is one of the economically most important bamboo species cultivated in the tropical countries. The present investigation is conceded to establish an effective method for clonal propagation of B. balcooa. The morphological response from nodal cuttings was tested on Murashige and Skoog’s (MS) basal medium mixed with a range of combinations and concentrations of 6-furfurylaminopurine (Kinetin) and 6-benzylaminopurine (BAP). About 96 % shoot sprouting was observed on medium containing 4.0 mg L⁻¹ BAP, 50 mg L⁻¹ ascorbic acid and 25 mg L⁻¹ each of l-arginine, citric acid and adenine sulphate. These shoots were proliferated efficiently on MS medium with 4.0 mg L⁻¹ BAP, 1.0 mg L⁻¹ NAA, which resulted in the formation of ∼62.0 shoots per node per culture bottle. In vitro rooting was achieved on ½-strength MS basal medium with 6.0 mg L⁻¹ NAA and 100 mg L⁻¹ activated charcoal. About one-month-old well-rooted plantlets were exposed to the greenhouse for 4 weeks in soilrite®. The hardened plantlets exhibited 100 % survival success under the field environment after 3 months. The monomorphic pattern of inter simple sequence repeats (ISSR) and start codon targeted (SCoT) markers of in vitro raised plants matched with mother plants confirmed the genetic stability. Moreover, in vitro raised plantlest also showed histological similarties with mother plant. Hence, this method could be efficiently utilized for commercial-scale production of B. balcooa.
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