Co-occurrence of five Fusarium toxins in corn-Dried Distiller’s Grains with Solubles in Thailand and comparison of ELISA and LC-MS/MS for fumonisin analysis
2013
Tansakul, Natthasit | Jala, Prakorn | Laopiem, Sudtisa | Tangmunkhong, Prapeuk | Limsuwan, Sasithorn
Dried Distiller’s Grains with Solubles (DDGS), a by-product of bio-ethanol production from maize and other cereals, is increasingly used as a feed additive. In this study, five Fusarium toxins, including fumonisin B₁(FB₁), fumonisin B₂(FB₂), deoxynivalenol (DON), zearalenone (ZEN) and beauvericin (BEA) were quantified by LC-MS/MS in 59 corn-DDGS samples. In addition, the fumonisin level in 30 randomly selected-samples was compared using an ELISA detection technique. No sample was free from mycotoxin contamination, and 50.8 % of the samples were co-contaminated with all five mycotoxins. Moreover, toxin levels were generally high, with mean levels of 9 mg kg⁻¹FB₁, 6 mg kg⁻¹FB₂, 1.2 mg kg⁻¹DON, 0.9 mg kg⁻¹ZEN, and 0.35 mg kg⁻¹BEA. Maximum levels for FB₁(143 mg kg⁻¹) and FB₂(125 mg kg⁻¹) are of acute toxicological relevance. The ELISA method had a tendency to underestimate the fumonisin content when compared with LC-MS/MS. Finally, this is the first reported beauvericin contamination in corn-DDGS.
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