Alginate Lyase Produced by Filamentous Fungus Through Solid State Fermentation Using Sargassum from the Brazilian Coast
2022
dos Santos Silva, Margarete Cabral | De Farias Silva, Carlos Eduardo | dos Santos, Leandro Monteiro | Medeiros, Josimayra Almeida | Vieira, Rosana Correia | de Souza Abud, Ana Karla | Almeida, Renata Maria Rosas Garcia | Tonholo, Josealdo
This article aimed at studying semi-solid-state fermentation using filamentous fungus for alginate lyase production using residual macroalgae as a substrate. Sargassum was the macroalgae chosen, given its wide abundance in the Brazilian coast. Among the fungal strains tested, Cunninghamella echinulata proved to be the most promising species. This work initially studied the optimisation of a nutrient solution (NS) in terms of nitrogen content, having verified that an increasing in peptone concentration (5 g/L) and the addition of yeast extract (2 g/L) (denominated NS₂) led to a satisfactory fungal development, doubling alginate lyase production. Regarding moisture content, an increasing of enzyme production was verified when 65–75% of moisture content was used, with production peak reaching between 175 and 200 U/mL. The use of inoculum concentration between 2.10⁶ and 1.10⁷ spores/gbᵢₒₘₐₛₛ did not have a significant influence on alginate lyase production. Moreover, the addition of an inducer (sodium alginate) increased enzyme production by 43%, shifting enzyme activity peak from 175 to 250 U/ml, with mass biomass substitution between 16.66 and 33.33% (wₐₗgᵢₙₐₜₑ/wₜₒₜₐₗ). The results showed that higher NS₂ pH (7–8.5) accelerated the production peak, which was higher than in the assays carried out with lower pH. Finally, stability of the enzyme extract to freezing was verified.
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