A β-glucan from the alkaline extract of a somatic hybrid (PfloVv5FB) of Pleurotus florida and Volvariella volvacea: structural characterization and study of immunoactivation
2013
Maity, Kankan K. | Patra, Sukesh | Dey, Biswajit | Bhunia, Sanjoy K. | Mandal, Soumitra | Bahera, Birendra | Maiti, Tapas K. | Sikdar, Samir R. | Islam, Syed S.
A water soluble polysaccharide isolated from the alkaline extract of the somatic hybrid mushroom (PfloVv5FB), obtained through protoplast fusion between Pleurotus florida and Volvariella volvacea strains was found to contain d-glucose only. Structural investigation was carried out using acid hydrolysis, methylation analysis; periodate oxidation, and NMR studies (¹H, ¹³C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, and HMBC). On the basis of the above mentioned experiments the structure of the repeating unit of the polysaccharide was established as: This polysaccharide exhibited strong immunoactivation of macrophages, splenocytes as well as thymocytes.
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