Cytogenetic study of in vitro-derived bovine embryos
1999
Ocana-Quero, J.M. | Pinedo-Merlin, M. | Moreno-Millan, M.
A cytogenetic study of early bovine embryos (2-16 blastomeres) produced in vitro was conducted to determine the incidence of embryos carrying chromosome amonalies. The embryos were produced from immature oocytes matured in vitro and fertilized by sperm prepared using the Percoll density gradient method. Slides were prepared according to an 'air drying' technique and the chromosomal complement of embryos was studied by Giemsa-staining. Approximately 57% of prepared embryos were suitable for analysis. The results revealed that 18% of cytogenetically analyzed embryos presented chromosomal anomalities, including haploid (8%), aneuploidy (2%) and polyploidy (8%). Our results were compared to the results of other studies in cattle and other domestic animals.
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