Comparison of Anaerobic Methane Oxidation in Different Sediment Habitats of Dianchi Lake
2020
Li, Wei | Chen, Yue | Luo, Mingmo | Li, Chunyan | Zhao, Yonggui | Chen, Huai | Feng, Defeng | Hu, Bin
Anaerobic methane oxidation is a key process for methane reduction and nitrogen removal in eutrophic lakes. It is very important to know the distribution patterns of anaerobic methane oxidation activity and the related microbes in the typical plateau eutrophic lakes. Here, we aimed to characterize the anaerobic methane oxidation activity and to reveal the correlations to biological/non-biological factors along eutrophic gradients in Dianchi Lake. The anaerobic methane oxidation activity was analyzed by anaerobic incubation and gas chromatography. Candidatus Methylomirabilis was analyzed by Illumina Miseq 16S ribosomal RNA gene sequencing. The methane oxidation activities ranged from 1.71 to 5.07 μg/g day⁻¹ in Dianchi Lake. These activities differed significantly among the four zones (p = 0.000), with more activity in the high eutrophication zone (Caohai). The relative abundance of Candidatus Methylomirabilis ranged from 84.86 to 98.13%, with no significant changes evident in the four research zones in Dianchi Lake. The anaerobic methane oxidation activity was significantly positively related to TN, NO₃⁻-N, TC, and Candidatus Methylomirabilis abundance, and significantly negatively related to TP and pH. Thus, our study showed anaerobic methane oxidation occurred in Dianchi sediment and the activity of anaerobic methane oxidation was associated with the level of eutrophication.
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