Establishment of Pyrosequencing Technology to Detect White Spot Syndrome Virus (WSSV) in Cultured Aquatic Animals
2019
Tong, Guixiang | Yin, Weili | Wu, Xiangqing | Lin, Yong | Chen, Xiaohan | Liang, Wanwen | Huang, Quoqiu | Huang, Luanyu | Huang, Guanghua | Yang, Huizan | Sun, Tao | Wei, Xinxian | Li, Xiaozheng
This study aimed to establish pyrosequencing methods to detect white spot syndrome virus (WSSV). One pair of polymerase chain reaction (PCR) primers, and one pyrosequencing primer, were designed for WSSV. The pyrosequencing reaction system and conditions were optimized and a pyrosequencing method for detecting WSSV was successfully established. This method was able to specifically detect WSSV in eight viruses, with high sensitivity. The minimum detectable limit for nucleic acid was 23 copies/μL. The method was verified by detecting WSSV in 1881 batches of samples collected from domestic and imported shrimps. The detection results were more sensitive than conventional PCR. This research has therefore provided a new detection method for monitoring, and controlling aquatic animal virus diseases.
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