The induction and development of potato microtubers in vitro on media free of growth regulating substances
1989
Garner, N. | Blake, J.
Reliable microtuber production has been obtained by culturing nodal explants of potato on Murashige and Skoog medium without the addition of growth regulating substances. The use of sucrose at 8 %, as compared with 4 or 12%, advanced the initiation of tuberization and gave more and larger microtubers. A period of 1 month under 16 h days, followed by transfer to 8 h photoperiods gave most rapid microtuber development. A reduction in the total nitrogen supply or an increase in the ratio of ammonium to nitrate reduced the size and number of microtubers.
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