Sequence polymorphism and structural analysis of timothy grass pollen profilin allergen (Phl p 11)
1997
Asturias, J.A. | Arilla, M.C. | Bartolome, B. | Martinez, J. | Martinez, A. | Palacios, R.
Three cDNA clones encoding timothy grass pollen profilin (Phl p 11) were newly isolated. Comparison of the sequences of four cDNA clones, including a previously isolated clone, showed a low level of polymorphism. Isoelectrofocusing of highly purified timothy grass profilin indicated the existence of at least five isoforms. One recombinant profilin showed similar immunological properties to natural timothy grass profilin. Tertiary structure of Phleum pratense profilin was obtained by homology-based molecular modeling.
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