Development and Validation of an Analytical Method for Quantification of Fucose in Sargassum horneri Extracts for Standardization of Functional Ingredients
2023
Park, S.G. | Yang, H.K. | Kim, Y.K. | Chang, N.I. | Om, A.S.
This study developed and validated a high-performance liquid chromatography (HPLC) analysis method for the quantification of fucose in Sargassum horneri extracts. HPLC was performed using a YMC-Pack ODS-AQ column (250 mm×4.6 mm, 5 μm) with a flow rate of 1.0 mL/min at 40°C, and detection was conducted at a wavelength of 250 nm. The validation of the method was assessed by evaluating specificity, accuracy, precision, limit of detection, limit of quantitation, and linearity. With respect to specificity, the retention time of the fucose standard solution matched that of the fucose in the Sargassum horneri extract. The linearity exhibited a correlation coefficient (R2) above 0.998. The accuracy assessment revealed a recovery rate ranging from 94.0±2.5% to 95.6±0.4% for different concentrations. The overall relative standard deviation (%RSD) for precision was 0.94%, indicating good repeatability, and the mean value over three days of analysis was 9.69±0.05 mg/g with a %RSD of 0.47%, demonstrating reproducibility. The limits of detection and quantitation were determined as 2.04 μg/mL and 6.19 μg/mL, respectively. Based on these results, the developed HPLC analysis method for fucose in Sargassum horneri extract could be considered reliable and reproducible.
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