Biological evaluation of mimetic peptides as active molecules for a new and simple skin test in an animal model
2019
Montalvo, Grecia, E Barriga | Thomaz-Soccol, Vanete | Vandenberghe, Luciana, P S | Carvalho, Júlio, C | Bertrand, Emmanuel | Prado, Maria, R M | Bonatto, Sandro, L. | Vandenberghe, Luciana P.S. | Prado, Maria R.M. | Bonatto, Sandro J.R. | Carbonera Guedes, Deborah | Kuhn, Aline | Pasquali, Sbruzzi | Rasmussen Petterle, Ricardo | Vanete, Dra | Soccol, Thomaz | Guedes, Deborah, Carbonera | Pasquali, Aline, Kuhn Sbruzzi | Minozzo, João, Carlos | Petterle, Ricardo, Rasmussen | Soccol, Carlos, R | Soccol, Vanete, Thomaz | Guedes, Deborah Carbonera | Pasquali, Aline Kuhn Sbruzzi | Minozzo, João Carlos | Faulds, Craig | Petterle, Ricardo Rasmussen | Soccol, Carlos Ricardo | Soccol, Vanete Thomaz | Biodiversité et Biotechnologie Fongiques (BBF) ; Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM) | Centro de Biologia Genômica e Molecular ; Pontifícia Universidade Católica do Rio Grande do Sul [Brasil] = Pontifical Catholic University of Rio Grande do Sul [Brazil] = Université catholique pontificale de Rio Grande do Sul [Brésil] (PUC-RS)
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Show more [+] Less [-]English. A skin test is a widely used tool in diagnostic evaluations to investigate cutaneous leishmaniases (CL). The actual antigen (Montenegro skin test [MST] antigen) presents some difficulties that pertain to its manufacturing and validation. To contribute to overcoming this problem, we propose the application of new-generation molecules that are based on skin antigen tests. These antigens were obtained through biotechnology pathways by manufacturing synthetic mimetic peptides. Three peptides, which were selected by phage display, were tested as skin test antigens in an animal model (Cavia porcellus) that was immunized with Leishmania amazonensis or Leishmania braziliensis. The peptide antigens, individually (PA1, PA2, PA3) or in a mix (PAMix), promoted induration reactions at 48 and 72 h after the test was performed. The indurations varied from 0.5 to 0.7 cm. In the animals immunized with L. amazonensis, the PA3 antigen showed better results than the standard MST antigen. In animals immunized with L. braziliensis, two peptide antigens (PA2 and PAMix) promoted induration reactions for a longer period of time than the standard MST antigen. These results validate our hypothesis that peptides could be used as antigens in skin tests and may replace the current antigen for CL diagnosis.
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