Time dynamics o fthe<em> Bacillus cereus</em> exoproteome are shaped by cellular oxidation
2015
Madeira, Jean-Paul | Alpha-Bazin, Beatrice | Armengaud, J. | Duport, Catherine | Sécurité et Qualité des Produits d'Origine Végétale (SQPOV) ; Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
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Show more [+] Less [-]English. At low density,<em> Bacillus cereus</em>cells release a large variety of proteins into the extracellular medium when cultivated in pH-regulated, glucosecontaining minimal medium, either in the presence or absence of oxygen. The majority of these exoproteins are putative virulence factors, including toxin-related proteins. Here, <em>B.cereus</em> exoproteome time courses were monitored by nanoLC-MS/MS under low-oxidoreduction potential (ORP) anaerobiosis, high-ORP anaerobiosis, and aerobiosis, with a specific focus on oxidative-induced post-translational modifications of methionine residues. Principal component analysis (PCA) of the exoproteome dynamics indicated that toxin-related proteins were the most representative of the exoproteome changes, both in terms of protein abundance and their methionine sulfoxide (Met(O)) content. PCA also revealed an interesting interconnection between toxin-, metabolism-, and oxidative stress–related proteins, suggesting that the abundance level of toxin-related proteins, and their Met(O) content in the <em>B.cereus</em> exoproteome, reflected the cellular oxidation under both aerobiosis and anaerobiosis.
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