Humoral and non-specific immune responses in rainbow trout (Oncorhynchus mykiss) naturally exposed to and immunized with Streptococcus iniae
2011
اخلاقی, مصطفی | Akhlaghi, Mostafa | Hoseini, Arsalan | Nematollahi, Amin | Hoseini, Seyed Mohammad
In this study, immune responses against Streptococcus iniae infections in rainbow trout (Oncorhynchus mykiss) were analyzed. Anti-streptococcal antibody (ASA) levels, respiratory burst activity (RBA), phagocytic activity (PA) and phagocytic index (P index) were measured in kidney macrophages. Fish were allocated to three experimental groups (n = 50 per group): survivors of natural S. iniae infections, non-exposed healthy fish immunized by immersion with formalin-killed S. iniae bacterin and a control group of non-exposed healthy fish. Blood sera and kidney samples were collected from each group (n = 10 fish) at days 0, 15, 30, 45 and 65 post-immunization (p.i.). Analysis of ASA levels, RBA, PA and P index in survivors of Streptococcus infections revealed optical density (OD) values of 0.082-0.091, 0.089-0.091, 53-55 and 1.8-1.9 respectively. In the immunized non-exposed group, the ASApeak antibody level occurred at day 15 (mean OD=0.122) and declined significantly by day 65 (p
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