Designing B and T Cell Multi-Epitope Vaccine for Cross Protection Against Haemophilus Strains: An Immunoinformatics Approach
2021
Nikbakht Borojeni, Gholamreza | Abasabadi, Fatemeh | Abiri, Ramin | Alvandi, Amirhooshang | Salari, Farhad
BACKGROUND: Despite the availability of effective conjugate vaccines, Haemophilus influenza (HI) and Haemophilus somnus (HS) still result in enormous global morbidity in both human and cattle. Vaccines failure to protect against different strains can lead to the spread of Hemophilus infections. The absence of various epitopes from Haemophilus strains in existing vaccines is one of their weaknesses. Therefore, selection of a conserved and common set of proteins in the invasive strains of HI and HS is essential for predicting epitopes as potential vaccine candidate. OBJECTIVES: The aim of this study was to design an effective polyepitopic vaccine against invasive HI and HS strains using in silico approaches. METHODS: First, the protein sequences were retrieved from the databases and were aligned to determine the conserved areas with the Clustal omega software. Then, B and T cell epitopes identification was done for OapA, OMP6, PD, D15, IgA1 Protease and TbpA proteins using various immunoinformatic servers. The high ranked epitopes were selected from mentioned proteins. The selected epitopes were fused together by appropriate linkers. This designed construct was analyzed for physicochemical and structural characteristics using related servers. RESULTS: 6 TCD4+ and 3 B cell epitopes were selected to design the final construct from 6 common proteins. The immunoinformatics analysis revealed that the designed polyepitopic peptide is a safe, soluble, hydrophilic and thermostable antigen that could be a potential vaccine. CONCLUSIONS: The polyepitopic construct can be considered as a vaccine candidate against Haemophilus.
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