The Impact of Surfactant on Aquatic Ecosystems: A Study on Biochemical Alterations in Clarias gariepinus Induced with Linear Alkylbenzene Sulfonates
2024
Ikpesu, Thomas Ohwofasa
The toxicity of linear alkylbenzene sulfonates (LABs) to Clarias gariepinus was investigated. For 30 days, the fish were exposed to LABs at 0.00, 0.50, 1.00, 1.50, and 2.00 mg/L. After each trial period, one fish from each plastic tub was chosen and its heart was punctured for blood samples. The blood samples were then collected and deposited in pre-designated bottles for analysis. Following blood collection, a fish was dissected and its organs were extracted. The organs were preserved in liquid nitrogen at -25oC until they were analyzed. A portable refractometer was used to quantify total serum protein content. A microplate reader was used to measure reduced glutathione (GSH). Albumin was quantified using the Bromocresol Green albumin assay kit, whereas alanine aminotransferase activity was assessed colorimetrically. Subtracting albumin from protein concentration yielded the globulin content. On days 23 and 30, protein content corresponds positively with exposure length and differs significantly (p < 0.05) between the control and treatment groups. The activity of GSH reduced slightly but not significantly (p > 0.05). Significant variations in albumin and globulin (p < 0.05) only on day 30. AAS activity differs significantly (p < 0.05) between treatments and the control. This study demonstrated that LAB exposure can be harmful to human health. Because anthropogenic sources are the primary source of LAB exposure, authorities must implement strict mitigation measures to limit this risk.
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